TRPML 1 and regulated exocytosis
نویسنده
چکیده
Figure EV1. Localization and levels of amylase, actin, Rab5, and Rab6 and effect of organellar pH on exocytosis in wild-type and Trpml1 / acini. A–C Isolated acini from the pancreas (A) and the parotid glands (B) of wild-type and Trpml1 / mice were stained for amylase, actin, Rab5, and Rab6 as listed in the images. The graph in (C) shows the mean s.e.m. fluorescence intensity analyzed with ImageJ of at least eight clusters containing 10–20 cells from three mice of each line. D–F Ca oscillations evoked by 3 pM CCK in wild-type (D) and Trpml1 / (E) pancreatic acini treated with 10 mM NH4 , and the graph in (F) shows the mean s.e.m. of the frequency. G, H Amylase release from pancreatic (G) and parotid acini (H) treated with 10 mM NH4 + and stimulated with CCK8 (G) or isoproterenol (H). The results are the mean s.e.m. of three experiments with acini obtained from three mice of each line. *P < 0.05.
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